Urinastatin

Literature References: Acid-stable glycoprotein (pI 2.8), mol wt ~67,000 by gel filtration. Consists of a single polypeptide chain of 147 amino acid residues containing two Kunitz-type protease inhibitor domains, and two carbohydrate side chains. Normally present in serum and urine. Isoln from human urine: N. R. Shulman, J. Biol. Chem. 213, 655 (1955); and purification: G. J. Proksch, J. I. Routh, J. Lab. Clin. Med. 79, 491 (1972). Characterization: M. Balduyck et al., Eur. J. Biochem. 158, 417 (1986). Protease inhibition: H. Sumi, N. Toki, Proc. Soc. Exp. Biol. Med. 167 530 (1981). Determn by enzyme immunoassay: N. Nishino et al., Haemostasis 19, 112 (1989). Biodistribution and clinical kinetics: B.-M. Jönsson-Berling, K. Ohlsson, Scand. J. Clin. Lab. Invest. 51, 549 (1991). Protective effect in exptl pancreatitis: T. Hirano, T. Manabe, Arch. Surg. 128, 1322 (1993). Clinical evaluation vs ischemic myocardial injury: S. Shimai et al., Jpn. Circ. J. 53, 1144 (1989); vs postoperative hyperamylasemia: D. Korenaga et al., Eur. Surg. Res. 23, 214 (1991). Receptor binding studies: H. Kobayashi et al., J. Biol. Chem. 269, 20642 (1994). Review of early literature: H. J. Faarvang, Scand. J. Clin. Lab. Invest. 17, Suppl. 83, 11-77 (1965). Review of characterization as subunit of inter-a-trypsin inhibitor (inter-a-inhibitor): W. Gebhard, K. Hochstrasser in Proteinase Inhibitors, A. J. Barrett, G. Salvesen, Eds. (Elsevier, Amsterdam, 1986) pp 389-401; W. Gebhard et al., Biol. Chem. Hoppe-Seyler 371, Suppl., 13-22 (1990).