Title: Cameleons
CAS Registry Number: 205599-45-7
Additional Names: Yellow cameleons; YCs
Literature References: Engineered proteins designed to measure intracellular calcium concentrations via FRET. Characterized by four domains: a modified green fluorescent protein, q.v.; a calmodulin; a cyan fluorescent protein; the calmodulin binding peptide, M13. Prepn: A. Miyawaki et al., Nature 388, 882 (1997); modification: eidem, Proc. Natl. Acad. Sci. USA 96, 2135 (1999). Use in monitoring calcium changes at vesicle surface: E. Emmanouilidou et al., Curr. Biol. 9, 915 (1999); in the endoplasmic reticulum: R. Yu, P. M. Hinkle, J. Biol. Chem. 275, 23648 (2000). Use as a tool for nervous system function in model organisms: R. Kerr et al., Neuron 26, 583 (2000).
Use: Indicator for intracellular calcium. |