Alfimeprase

Literature References: Truncated form of fibrolase, q.v., containing 201 amino acid residues; mol wt ~22.6 kDa. Directly acting fibrinolytic metalloproteinase; preferentially degrades the Aa chain of fibrinogen, q.v. Inhibited by serum a2-macroglobulin. Prepn by recombinant technology in Pichia pastoris: T. C. Boone et al., WO 0125445; eidem, US 6261820 (both 2001 to Amgen). Disulfide structure: G. Jones et al., Protein Sci. 10, 1264 (2001). Pharmacology: C. F. Toombs, Haemostasis 31, 141 (2001). Review of biochemistry and pharmacology: idem, Fundam. Clin. Cardiol. 46, 627-650 (2003). Clinical evaluation for peripheral arterial thrombolysis: K. Ouriel et al., J. Vasc. Interv. Radiol. 16, 1075 (2005).